Poly-l-gamma-glutamic acid production by recombinant Bacillus subtilis without pgsA gene
نویسندگان
چکیده
منابع مشابه
Improvement of Bacillus subtilis for poly‐γ‐glutamic acid production by genome shuffling
Poly-γ-glutamic acid (γ-PGA) is a promising microbial polymer with potential applications in industry, agriculture and medicine. The use of high γ-PGA-producing strains is an effective approach to improve productivity of γ-PGA. In this study, we developed a mutant, F3-178, from Bacillus subtilis GXA-28 using genome shuffling. The morphological characteristics of F3-178 and GXA-28 were not ident...
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A bacterial strain of marine origin showing production of poly(g-glutamic acid) (PGA) has been identified by taxonomical and 16S rRNA studies as Bacillus subtilis. A sequential optimization approach was applied for improving the PGA production. The effect of carbon sources, nitrogen sources and pH on the production of PGA was investigated by one factor-at-a-time method. Plackett-Burman design w...
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Many studies have clarified that poly(γ-glutamic acid) (PGA) increases the solubility of Ca 2+ , suggesting that PGA enhances calcium absorption in the small intestine. However, there has been no report on the specific interaction between PGA and Ca 2+ in water. We studied the aqueous solution properties of PGA calcium salt (PGA-Ca complex). The chelating ability and binding strength of PGA for...
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Bacillus licheniformis ATCC 9945a is one of the bacterial strains that produce gamma-poly(glutamic acid) (gamma-PGA). The use of carbohydrate medium components for gamma-PGA production was explored. Cells were grown in shake flasks or in controlled pH fermentors using medium formulations that contain different carbon sources. During the cultivations, aliquots were removed to monitor cell growth...
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ژورنال
عنوان ژورنال: AMB Express
سال: 2018
ISSN: 2191-0855
DOI: 10.1186/s13568-018-0636-x